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Subject: Johns Hopkins Joins 20th Century (L-forms of Lyme bacteria
resistant to antibiotics, duh)
Date: Nov 6, 2009 7:01 AM
Well, well, well.
Thank you for joining us:
http://www.actionlyme.org/RICOCHRON.htm
http://www.actionlyme.org/BOGUS_RUSSIAN_NYMC_ARTICLES.htm
http://www.actionlyme.org/IDSA_CYST_VIABLE.htm
http://www.actionlyme.org/CHP_9_IDSA_REVIEWS.htm
"Reversion of L-Forms to intact sphirochetes
may take *months* in culture"- Russell Johnson:
http://www.actionlyme.org/IDSA_JOHNSONCULTURING.htm
and not "2-3 weeks," like Marky-Famous
Bioweaponer-Bumbler-of-Downtown-Boston
sez:
http://www.actionlyme.org/MKLEMPNER.htm
LMAO.
*NOW* how is IDsociety.org gonna say "Lyme
is cured in month or else it's 'psychiatric?'"
JH says cysts are real, and Tufts is
about to transfer them as infected, treated
spinal fluid to uninfected mouse CSF.
This is hilarious.
This is a 1950s-1970s *FACT*:
http://www.actionlyme.org/RICOCHRON.htm
DA-AMN, I thought the funniest things
to ever happen on earth, already happened.
Now JH is gonna do 1950s science and
call that a "discovery."
Here are all published articles from
Jan 1, 1900 to Jan 1, 1970 (over 500)
http://www.ncbi.nlm.nih.gov/sites/entrez?db=pubmed&cmd=DetailsSearch&term=%28%221900%2F01%2F01%22[Publication+Date]+%3A+%221970%2F01%2F01%22[Publication+Date]%29+AND+%28L-forms%29&log$=activity
Ya see, so I am not exaggerating
that this is ancient history. All
this real, actual, true, well-known
science was "forgotten" once that
idiot draft-dodger fairy Allen Steere
bumbled and stumbled into "medicine"
to avoid VietNam and decided he
would be famous and a millionaire
and have a disease named after
himself...
Gross.
Kathleen M. Dickson
http://www.actionlyme.org
http://www.relapsingfever.org
=====================================
http://media.www.jhunewsletter.com/media/storage/paper932/news/2009/11/05/ScienceTech/Bacterial.Antibiotic.Resistance.Genes.Discovered-3824803.shtml
Bacterial antibiotic resistance genes discovered
By Aleena Lakhanpal
Issue date: 11/5/09
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Antibacterial soap, hand sanitizer and antibiotics are all substances
that we use in an attempt to kill bacteria that might make us
sick.Whether we are concerned about getting strep throat, bacterial
meningitis or something else, these prevention methods can offer
protection.
However, some bacteria, such as those that cause Staph and MRSA
infections, are becoming increasingly resistant to antibiotics. Since
the 1930s, researchers have been aware that bacteria may be able to
resist treatment because they can morph into the L-form, or bacteria
lacking cell walls.
Until the 1980s, not much else could be known about the L-form, but
now, researchers at the Bloomberg School of Public Health have used a
wide variety of modern molecular tools to learn more about the origin
and biological functions of the L-form bacteria.
Ying Zhang, a professor of molecular microbiology and immunology at
Bloomberg, is the senior author of the study, which was published in
PLoS ONE last month.
Not all bacteria can transform into the L-form, but those that can
include Bacillus anthracis (anthrax), Treponema pallidum (syphilis),
Mycobacterium tuberculosis (tuberculosis), Heliobacter pylori (stomach
ulcers and cancer), Borrelia burgdorferi (Lyme disease) and
Escherichia coli (food poisoning). Zhang's team used E. coli to create
a culture of L-form bacteria.
Although it had been difficult to culture L-form bacteria before,
Zhang and his team created a new method that more closely simulated
the in vivo conditions in which these bacteria form.
"The presence of antibiotic stress is cell wall inhibiting, like
penicillin," Zhang said. To prevent the cells from bursting because of
this increased stress, Zhang's team added sucrose to the cell media.
This culture represented the mechanism that occurs in the body. "L
forms are formed in response to stress," Zhang said. "They have a
different mode of survival and replication from classical bacteria."
The cell wall-deficient bacteria cluster together in the shape of a
fried egg rather than the smooth, homogeneous appearance of wild-type
bacteria cultures.
Continued...
Not only are L-form bacteria difficult to culture and therefore study,
but this "fried egg" cluster is part of what makes the L-form bacteria
resistant to antibiotics, in addition to the fact that they do not
have cell walls for commonly used antibiotics to disintegrate.
Once Zhang and his team were able to successfully culture L-form E.
coli, they screened for and identified mutants that fail to grow at
the L-form. From these mutants, they were able to discover a series of
genes that were linked with the inability to grow in the L-form.
"These fall into four to five different categories involving
extracellular matrix synthesis, membrane proteins, membrane
biogenesis, DNA repair as well as iron metabolism and energy
metabolism," Zhang said.
Their identification of these genes and their effect on L-form
bacterial expression is a resounding discovery because it was
impossible to do before, what with the difficulty of culturing the L-
forms of various bacteria. Zhang noted, however, that although his
team managed to create and study a culture of L-form bacteria, their
study cannot be universal.
"What we can culture is only a small percentage - probably less than 1
percent - of all bacteria on earth," Zhang said.
"They exist in nature and grow easily, but we're limited to what we
can grow and the form of bacteria that can grow. Bacteria can grow a
variety of different forms even for the same species, and can change
forms under different conditions. L-forms are one example of changing
under antibiotic stress."
These L-forms of various bacteria may be the underlying reason for
chronic resistant and recurring diseases, such as sarcoidosis, various
forms of inflammatory bowel diseases and rheumatoid arthritis. Zhang
is confident that there will be many practical applications of this
discovery.
"It is possible, with our discovery of the L-form genesĀ to develop
new antibiotics and more effective ones that can be used with current
ones as well as new vaccines to . . . allow these forms to be
eliminated by the immune system," he said.
"[Real] scientists are *fiercely* independent. That's the good
news."-- NIH's Top Fool, Anthony Fauci |
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