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Science Forum Index » Medicine - Cancer Forum » Genistein, DHA strongly synergistic against breast cancer
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| Steve |
 Posted: Tue Mar 11, 2008 11:21 pm |
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Guest
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LEF (www.lef.org or maybe it's dot com) sells genistein pills with lots of
genistein in each one. DHA is available at any vitamin store (only buy it if
it's in the frig and keep it in the frig after you buy it).
Here's the link to the whole paper (free) and the abstract:
http://carcin.oxfordjournals.org/cgi/content/full/28/4/809
Carcinogenesis. 2007 Apr;28(4):809-15. Epub 2006 Oct 19.
Complementary actions of docosahexaenoic acid and genistein on COX-2, PGE2
and invasiveness in MDA-MB-231 breast cancer cells.
Horia E, Watkins BA.
Center for Enhancing Foods to Protect Health, Lipid Chemistry and Molecular
Biology Laboratory, Purdue University, West Lafayette, IN 47907, USA.
N-3 polyunsaturated fatty acids (PUFA) and genistein have been associated
with lowered cancer risk by reducing inflammatory prostanoids,
cyclooxygenase-2 (COX-2) activity, and altering cell signaling. Few studies
have investigated the effect of these compounds in combination on the
molecular control of the COX-2 gene. In a series of experiments we examined
a potential synchronous action of n-3 PUFA and genistein in down-regulating
COX-2 expression to diminish prostaglandin E(2) (PGE(2)) production in
MDA-MB-231 human breast cancer cells. Cells were treated with genistein and
various PUFA including arachidonic acid (AA), eicosapentaenoic acid (EPA),
and docosahexaenoic acid (DHA). PGE(2) concentrations, expression of COX-2,
and cell invasiveness were determined. The n-3 PUFA and genistein alone
lowered PGE(2) concentration, and genistein in combination with AA reversed
the high level of this prostanoid in cell cultures enriched with AA. The
degree of cell invasiveness was reversed by genistein in cell cultures
treated with AA and further reduced in those given DHA. The n-3 PUFA, in
contrast to AA, reduced COX-2 and NFkappaB expression. Genistein combined
with AA reversed the effects of AA alone on the expression of COX-2 and
NFkappaB. All three fatty acids increased the expression of PPARgamma in the
cells only when combined with genistein. Our results support the premise
that DHA and genistein exert complementary actions whilst genistein is
antagonistic to AA for controlling PGE(2) production as well as invasiveness
of MDA-MB-231 cells in culture by modulating the level of NFkappaB
expression. |
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