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Science Forum Index » Optics Forum » Microscope question
Page 1 of 1
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Message |
| Jorge Posada |
Posted: Fri Nov 07, 2003 11:06 am |
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Guest
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Hello
I apologize if this seems an obvious question, but i would be really
grateful for any hint.
In cancer diagnosis typically a 2x2 cm probe is magnified -using a
microsope- between 10x and 400x (of course for a small subsection of
the probe)
I would restrict my question to a 1x1 cm subsection of such a probe.
Now my question: Is it possible to make by a suitable optical
arrangement of lens, lights, projection mechanism, etc. attached to
the microscope, so that the whole 1 x 1 cm subsection is amplified
and projected with 100x in a screen of 1 x1 meters? Does this make
sense?
Thanks a lot,
Jorge Posada |
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| Jürgen Appel |
Posted: Fri Nov 07, 2003 6:04 pm |
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Guest
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Jorge Posada schrieb:
Quote: Now my question: Is it possible to make by a suitable optical
arrangement of lens, lights, projection mechanism, etc. attached to
the microscope, so that the whole 1 x 1 cm subsection is amplified
and projected with 100x in a screen of 1 x1 meters? Does this make
sense?
Yes, it is possible, but the projection would get quite dark, since all the
light that goes to the screen has to pass through the 1 cm crossection, so
at the probe's plane the intensity is 10000 times the intensity on the
screen.
It would probably be easier to use a digital camera and a video projector.
Then you would have the advantage to easily acquire digital images for the
records.
Regards,
Jürgen
--
GPG key:
http://pgp.mit.edu:11371/pks/lookup?search=J%FCrgen+Appel&op=get |
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| mg |
Posted: Fri Nov 07, 2003 11:14 pm |
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Guest
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Regular microscope -> digital camera attachment -> LCD or DLP projector
"Jorge Posada" <jorge.posada@excite.com> wrote in message
news:362ca890.0311070806.5ef7e9f0@posting.google.com...
Quote: Hello
I apologize if this seems an obvious question, but i would be really
grateful for any hint.
In cancer diagnosis typically a 2x2 cm probe is magnified -using a
microsope- between 10x and 400x (of course for a small subsection of
the probe)
I would restrict my question to a 1x1 cm subsection of such a probe.
Now my question: Is it possible to make by a suitable optical
arrangement of lens, lights, projection mechanism, etc. attached to
the microscope, so that the whole 1 x 1 cm subsection is amplified
and projected with 100x in a screen of 1 x1 meters? Does this make
sense?
Thanks a lot,
Jorge Posada |
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| Back to top |
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| A Norton |
Posted: Sat Nov 08, 2003 2:57 am |
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Guest
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Simply magnifying the image by 100X will not give you the same resolution as
a 100X microscope objective. The resolution is largely determined by the
numerical aperture (NA) of the objective which is the sine of the maximum
angle collected multiplied by the refractive of either index of air or
immersion oil whichever is used. A 100X objective might have an NA of 0.8
to 0.9. The problem is these types of objective lenses have a field size of
only about 0.15 mm. A lens that would have this NA and a 10mm field would
be really huge and expensive. The closest thing would be the lenses used to
print patterns on integrated circuits. These cost about a million dollars
each just for the lens assembly.
-Adam Norton
"Jorge Posada" <jorge.posada@excite.com> wrote in message
news:362ca890.0311070806.5ef7e9f0@posting.google.com...
Quote: Hello
I apologize if this seems an obvious question, but i would be really
grateful for any hint.
In cancer diagnosis typically a 2x2 cm probe is magnified -using a
microsope- between 10x and 400x (of course for a small subsection of
the probe)
I would restrict my question to a 1x1 cm subsection of such a probe.
Now my question: Is it possible to make by a suitable optical
arrangement of lens, lights, projection mechanism, etc. attached to
the microscope, so that the whole 1 x 1 cm subsection is amplified
and projected with 100x in a screen of 1 x1 meters? Does this make
sense?
Thanks a lot,
Jorge Posada |
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| Back to top |
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| Jorge Posada |
Posted: Tue Nov 11, 2003 9:20 am |
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Guest
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Hello,
Thank you for the very complete answer!
Just a last question: what could be the effect of doing it with a bad
resolution, with a low NA? What could be the consequences for the
image in the projection screen? Will it be dark, or ill defined, or
blurry?
Thanks a lot again!
Jorge POsada
"A Norton" <anortonREMOVETHIS@ix.netcom.com> wrote in message news:<am1rb.1955$6c3.1302@newsread1.news.pas.earthlink.net>...
Quote: Simply magnifying the image by 100X will not give you the same resolution as
a 100X microscope objective. The resolution is largely determined by the
numerical aperture (NA) of the objective which is the sine of the maximum
angle collected multiplied by the refractive of either index of air or
immersion oil whichever is used. A 100X objective might have an NA of 0.8
to 0.9. The problem is these types of objective lenses have a field size of
only about 0.15 mm. A lens that would have this NA and a 10mm field would
be really huge and expensive. The closest thing would be the lenses used to
print patterns on integrated circuits. These cost about a million dollars
each just for the lens assembly.
-Adam Norton
"Jorge Posada" <jorge.posada@excite.com> wrote in message
news:362ca890.0311070806.5ef7e9f0@posting.google.com...
Hello
I apologize if this seems an obvious question, but i would be really
grateful for any hint.
In cancer diagnosis typically a 2x2 cm probe is magnified -using a
microsope- between 10x and 400x (of course for a small subsection of
the probe)
I would restrict my question to a 1x1 cm subsection of such a probe.
Now my question: Is it possible to make by a suitable optical
arrangement of lens, lights, projection mechanism, etc. attached to
the microscope, so that the whole 1 x 1 cm subsection is amplified
and projected with 100x in a screen of 1 x1 meters? Does this make
sense?
Thanks a lot,
Jorge Posada |
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| Back to top |
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| A Norton |
Posted: Wed Nov 12, 2003 2:04 am |
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Guest
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You could probably come up with an illumination system that would let you
project a 10X10mm field onto the wall with reasonable brightness (think of a
slide projector). However, features that are below the resolution limt of
the objective will simply blur together.
"Jorge Posada" <jorge.posada@excite.com> wrote in message
news:362ca890.0311110620.55679539@posting.google.com...
Quote: Hello,
Thank you for the very complete answer!
Just a last question: what could be the effect of doing it with a bad
resolution, with a low NA? What could be the consequences for the
image in the projection screen? Will it be dark, or ill defined, or
blurry?
Thanks a lot again!
Jorge POsada
"A Norton" <anortonREMOVETHIS@ix.netcom.com> wrote in message
news:<am1rb.1955$6c3.1302@newsread1.news.pas.earthlink.net>...
Simply magnifying the image by 100X will not give you the same
resolution as
a 100X microscope objective. The resolution is largely determined by
the
numerical aperture (NA) of the objective which is the sine of the
maximum
angle collected multiplied by the refractive of either index of air or
immersion oil whichever is used. A 100X objective might have an NA of
0.8
to 0.9. The problem is these types of objective lenses have a field
size of
only about 0.15 mm. A lens that would have this NA and a 10mm field
would
be really huge and expensive. The closest thing would be the lenses
used to
print patterns on integrated circuits. These cost about a million
dollars
each just for the lens assembly.
-Adam Norton
"Jorge Posada" <jorge.posada@excite.com> wrote in message
news:362ca890.0311070806.5ef7e9f0@posting.google.com...
Hello
I apologize if this seems an obvious question, but i would be really
grateful for any hint.
In cancer diagnosis typically a 2x2 cm probe is magnified -using a
microsope- between 10x and 400x (of course for a small subsection of
the probe)
I would restrict my question to a 1x1 cm subsection of such a probe.
Now my question: Is it possible to make by a suitable optical
arrangement of lens, lights, projection mechanism, etc. attached to
the microscope, so that the whole 1 x 1 cm subsection is amplified
and projected with 100x in a screen of 1 x1 meters? Does this make
sense?
Thanks a lot,
Jorge Posada |
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