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Science Forum Index » Life Extension Forum » Inhibition of lipoxygenases and cyclooxygenase-2...
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| rs1000b at (no spam) yahoo.com... |
 Posted: Sat Jun 07, 2008 7:42 am |
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J Ethnopharmacol. 2008 Apr 24. [Epub ahead of print]
Inhibition of lipoxygenases and cyclooxygenase-2 enzymes by extracts
isolated from Bacopa monniera (L.)
Wettst.Viji V, Helen A.
Department of Biochemistry, University of Kerala, Kariavattom,
Thiruvananthapuram, Kerala 695 581, India.
AIM OF THE STUDY: Bacopa monniera Linn is described in the Ayurvedic
Materia Medica, as a therapeutically useful herb for the treatment of
inflammation. In the current study, we investigated the anti-
inflammatory activity of methanolic extract of Bacopa monniera (BME).
For some experiments EtOAc and bacoside fractions were prepared from
BME. The effect of these extracts in modulating key mediators of
inflammation was evaluated. MATERIALS AND METHODS: Carrageenan-induced
rat paw edema, rat mononuclear cells and human whole blood assay were
employed as in vivo and in vitro models. RESULTS: In carrageenan-
induced rat paw edema, BME brought about 82% edema inhibition at a
dose of 100mg/kg i.p. when compared to indomethacin (INDO) (3mg/kg)
that showed 70% edema inhibition. BME also significantly inhibited 5-
lipoxygenase (5-LOX), 15-LOX and cyclooxygenase-2 (COX-2) activities
in rat monocytes in vivo. Among the fractions tested in vitro, EtOAc
fraction possessed significant 1,1-diphenyl-2-picrylhydrazyl radical
scavenging activity with IC(50) value of 30mug/ml compared to
butylated hydroxyl toluene (IC(50)=13mug/ml). This fraction also
exerted significant hydroxyl radical scavenging activity with IC(50)
value of 25mug/ml in comparison with quercetin (IC(50)=5mug/ml).
Inhibitory effects of EtOAc and bacoside fractions on LOX and COX
activities in Ca-A23187 stimulated rat mononuclear cells were also
assessed. 5-LOX IC(50) values were 25mug/ml for EtOAc, 68mug/ml for
bacosides and 2mug/ml for nordihydroguaiaretic acid (NDGA) where as
COX-2 IC(50) values were 1.32mug/ml for EtOAc, 1.19mug/ml for bacoside
fraction and 0.23mug/ml for indomethacin. EtOAc and bacoside fractions
also brought about significant decrease in TNF-alpha release ex vivo.
CONCLUSION: Bacopa monniera possesses anti-inflammatory activity
through inhibition of COX and LOX and downregulation of TNF-alpha.
PMID: 18534796 [PubMed - as supplied by publisher] |
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