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Science Forum Index » Medicine - Nursing Forum » Detecting Degenerating Brain Disease...
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| ironjustice... |
 Posted: Sat Jul 12, 2008 7:16 pm |
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" Iron storage protein ferritin for detecting degenerating microglia"
Microglial dystrophy in the aged and Alzheimer's disease brain is
associated with ferritin immunoreactivity.
Aug 1, 2008
Kryslaine O Lopes,D Larry Sparks,Wolfgang J Streit
Degeneration of microglial cells may be important for understanding
the pathogenesis of aging-related neurodegeneration and
neurodegenerative diseases.
In this study, we analyzed the morphological characteristics of
microglial cells in the nondemented and Alzheimer's disease (AD) human
brain using ferritin immunohistochemistry.
The central hypothesis was that expression of the iron storage protein
ferritin increases the susceptibility of microglia to degeneration,
particularly in the aged brain since senescent microglia might become
less efficient in maintaining iron homeostasis and free iron can
promote oxidative damage.
In a primary set of 24 subjects (age range 34-97 years) examined,
microglial cells immunoreactive for ferritin were found to constitute
a subpopulation of the larger microglial pool labeled with an antibody
for HLA-DR antigens.
The majority of these ferritin-positive microglia exhibited aberrant
morphological (dystrophic) changes in the aged and particularly in the
AD brain.
No spatial correlation was found between ferritin-positive dystrophic
microglia and senile plaques in AD tissues.
Analysis of a secondary set of human postmortem brain tissues with a
wide range of postmortem intervals (PMI, average 10.94 +/- 5.69 h)
showed that the occurrence of microglial dystrophy was independent of
PMI and consequently not a product of tissue autolysis.
Collectively, these results suggest that microglial involvement in
iron storage and metabolism contributes to their degeneration,
possibly through increased exposure of the cells to oxidative stress.
We conclude that ferritin immunohistochemistry may be a useful method
for detecting degenerating microglia in the human brain.
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