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Science Forum Index » Chemistry Forum » IEP/PZC of adrenalin, cortisol?...
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| MinnRB... |
 Posted: Thu Jun 05, 2008 10:30 am |
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I'm a chemistry novice trying to find charge/IEP of adrenalin,
cortisol, glucose/glucagon, estrogen, cytokines etc. I have access to
the libraries of a major university. Found Insulin in a bioChemistry
dictionary, (weak acid with an IEP of pH/5.4). I've looked thru
medical school bioChemistry textbooks and all over the web but I'm
unsuccessful. Can anyone point me to a source/sources?
Am I not trying hard enough? Thanks. |
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| Bob... |
| Posted: Thu Jun 05, 2008 11:34 pm |
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On Thu, 5 Jun 2008 13:30:39 -0700 (PDT), MinnRB <barron.rj at (no spam) gmail.com>
wrote:
Quote: I'm a chemistry novice trying to find charge/IEP of adrenalin,
cortisol, glucose/glucagon, estrogen, cytokines etc.
Where IEP apparently means isoelectric point.
Your list of chemicals is a mix of small molecules and proteins.
For the small molecules, just go look at the structures. Glucose has
no ionizable groups, hence no charge, period. So the concept of IEP
isn't even relevant.
I'd be surprised if anyone measures IEP of small molecules.
I don't even know that they do it often for proteins.
There are programs that will estimate the IEP based on the amino acid
composition, assuming that each amino acid behaves normally. Since
most do, the programs end up being fairly close.
What kinds of searches have you done?
I suppose I might try something like
isoelectric focusing proteins
or
isoelectric point <name of specific protein>
Why do you care, esp if you are a novice?
bob
Quote: I have access to
the libraries of a major university. Found Insulin in a bioChemistry
dictionary, (weak acid with an IEP of pH/5.4). I've looked thru
medical school bioChemistry textbooks and all over the web but I'm
unsuccessful. Can anyone point me to a source/sources?
Am I not trying hard enough? Thanks. |
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