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Science Forum Index » Life Extension Forum » Aminoguanidine delays the replicative senescence of...
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| rs1000b at (no spam) yahoo.com... |
 Posted: Sun May 11, 2008 11:43 am |
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Chin Med J (Engl). 2007 Nov 20;120(22):2028-35.
Aminoguanidine delays the replicative senescence of human diploid
fibroblasts.
Wang PC, Zhang J, Zhang ZY, Tong TJ.
Department of Medical Laboratory, Xuanwu Hospital, Capital Medical
University, Beijing 100053, China. peichangwang at (no spam) at (no spam) at (no spam) yahoo.com
BACKGROUND: The accumulation of free radicals and advanced glycation
end products (AGEs) in cell plays a very important role in replicative
senescence. Aminoguanidine (AG) has potential antioxidant effects and
decreases AGE levels. This study aimed to investigate its effect on
replicative senescence in vitro. METHODS: The effects of
aminoguanidine on morphology, replicative lifespan, cell growth and
proliferation, AGEs, DNA damage, DNA repair ability and telomere
length were observed in human fetal lung diploid fibroblasts (2BS).
RESULTS: Aminoguanidine maintained the non-senescent phenotype of 2BS
cells even at late population doubling (PD) and increased cumulative
population doublings by at least 17 - 21 PDs. Aminoguanidine also
improved the potentials of growth and proliferation of 2BS cells as
detected by the MTT assay. The AGE levels of late PD cells grown from
early PD in DMEM containing aminiguanidine decreased significantly
compared with those of late PD control cells and were similar to those
of young control cells. In addition, the cells pretreated with
aminoguanidine had a significant reduction in DNA strand breaks when
they were exposed to 200 micromol/L H(2)O(2) for 5 minutes which
indicated that the compound had a strong potential to protect genomic
DNA against oxidative stress. And most of the cells exposed to 100
micromol/L H(2)O(2) had much shorter comet tails and smaller tail
areas after incubation with aminoguanidine-supplemented DMEM, which
indicated that the compound strongly improved the DNA repair abilities
of 2BS cells. Moreover, PD55 cells grown from PD28 in 2 mmol/L or 4
mmol/L aminoguanidine retain telomere lengths of 7.94 kb or 8.12 kb,
which was 0.83 kb or 1.11 kb longer than that of the control cells.
CONCLUSION: Aminoguanidine delays replicative senescence of 2BS cells
and the senescence-delaying effect of aminoguanidine appear to be due
to its many biological properties including its potential for
proliferation improvement, its inhibitory effect of AGE formation,
antioxidant effect, improvement of DNA repair ability and the slowdown
of telomere shortening.
PMID: 18067791 [PubMed - indexed for MEDLINE] |
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