| |
 |
|
|
Science Forum Index » Medicine - Lyme Forum » Steere 2007: C6 ELISA may not cover all LB cases
Page 1 of 1
|
| Author |
Message |
| CaliforniaLyme |
 Posted: Sat Jan 20, 2007 11:01 am |
|
|
|
Guest
|
1: Int J Med Microbiol. 2007 Jan 16; [Epub ahead of print]Immune
responses to borrelial VlsE IR(6) peptide variants.
Sillanpaa H, Lahdenne P, Sarvas H, Arnez M, Steere A, Peltomaa M,
Seppala I.
Department of Bacteriology and Immunology, Haartman Institute,
Helsinki, Finland.
Laboratory confirmation of Lyme borreliosis (LB) relies mainly on the
demonstration of anti-borrelial antibodies. In recent studies, a novel
VlsE protein IR(6) peptide-based assay has been introduced. Our aim was
to evaluate the IR(6) peptides from three Borrelia burgdorferi sensu
lato genospecies in the serodiagnosis of European and North American
patients. Five VlsE protein IR(6) peptide variants representing
sequences from B. burgdorferi sensu stricto, B. garinii, and B. afzelii
were used as antigens in both IgG and IgM enzyme-linked immunosorbent
assays (ELISA). Serum antibodies of 187 patients at different stages of
LB from Europe and the United States were evaluated for serodiagnosis.
For comparison samples were tested with one of the commercial IR(6)
ELISAs. Three B. afzelii IR(6) variant peptides revealed antibodies
that were concordant with each other. B. burgdorferi sensu stricto
peptide antibodies mostly paralleled B. afzelii peptide antibodies, and
positive values were also obtained in the majority of European sera.
For several sera, B. garinii IR(6) peptide antibodies were discordant
to B. afzelii peptide antibodies. The commercial IR(6) peptide antibody
assay (C6 ELISA) results correlated better with B. burgdorferi sensu
stricto IR(6) than with B. garinii IR(6) peptide IgG results,
especially in sera from patients with facial palsy. Thus, antibody
specificity to IR(6) peptides may vary according to the infecting
Borrelia species. In some manifestations of the disease, C6 ELISA may
not cover all LB cases. Evidently, the methodological aspects in ELISA
design for peptide antibody measurements are important as well as the
amino acids sequence of the antigen.
PMID: 17234451 [PubMed - as supplied by publisher] |
|
|
| Back to top |
|
| |
|
Page 1 of 1
All times are GMT - 5 Hours
The time now is Mon Oct 13, 2008 4:09 am
|
|