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Science Forum Index » Life Extension Forum » Antiapoptotic effects of pyruvate in ischemia
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| kofi |
 Posted: Mon Jan 12, 2004 6:45 pm |
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Transplantation. 2001 Jul 15;72(1):27-30. Related Articles, Links
Comment in:
* Transplantation. 2002 Jun 15;73(11):1851.
Click here to read
Pyruvate inhibits hepatic ischemia-reperfusion injury in rats.
Sileri P, Schena S, Morini S, Rastellini C, Pham S, Benedetti E,
Cicalese L.
Transplant Division, University of Illinois at Chicago, USA.
BACKGROUND: Ischemia/reperfusion (I/R) injury is a limiting factor
in liver transplantation. We have recently shown that pyruvate (PY)
inhibits intestinal and renal I/R injury. This study aims to evaluate
the protective effect of PY on hepatic I/R injury. METHODS: ACI rats
were treated with PY, whereas control animals received placebo. Rats
were killed after 60 min of partial hepatic ischemia and after 2, 6, 24,
and 48 hr of reperfusion. For each time point, serum aspartate
aminotransferase, alanine aminotransferase, and lactate dehydrogenase
were measured, and liver biopsy specimens were obtained to evaluate
morphology, DNA fragmentation, and apoptosis (terminal deoxynucleotidyl
transferase-mediated deoxyuridine triphosphate-biotin nick end
labeling). RESULTS: The survival rate 48 hr after I/R was 83% in the
control group, and 100% in the PY-treated group (P>0.05). Increased
enzymatic levels and histologic findings showed increased liver damage
in the untreated group compared with PY. In control rats, apoptosis was
enhanced after 1 hr of ischemia and peaked after 2 hr of reperfusion, to
decrease gradually 48 hr after reperfusion; in the PY group apoptosis
was delayed and reduced. After 1 hr of ischemia, the number of apoptotic
nuclei was significantly increased in control livers compared with
normal preischemic livers, whereas the number was significantly reduced
by PY. After 2 hr of reperfusion, the maximum number of apoptotic cells
was observed, whereas PY significantly reduced the amount of apoptotic
cells (P<0.05). Apoptosis was delayed in PY-treated livers to 6 hr after
reperfusion, peaking at a significantly lower count compared with
placebo-treated controls (P<0.05). CONCLUSION: These data indicate that
PY has a protective effect on I/R injury of the liver.
PMID: 11468530 [PubMed - indexed for MEDLINE] |
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