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Science Forum Index » Life Extension Forum » Effects of biotin on pyruvate
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| kofi |
 Posted: Mon Jan 12, 2004 6:43 pm |
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J Nutr. 2003 Sep;133(9):2857-62. Related Articles, Links
Click here to read
Pharmacological biotin supplementation maintains biotin status and
function in rats administered dietary carbamazepine.
Rathman SC, Gregory JF 3rd, McMahon RJ.
Center for Nutritional Sciences, University of Florida, Gainesville,
FL 32611, USA.
Biotin status and function are decreased during oral carbamazepine
(CBZ) administration in both humans and rats, but it is not known
whether biotin supplementation can prevent these decreases. To test the
effectiveness of pharmacologic biotin supplementation during CBZ
administration, 55 rats were randomly divided into 4 groups (0.06 mg
biotin/kg diet +/- 3.75 g CBZ/kg diet and 6.0 mg biotin/kg diet +/- 3.75
g CBZ/kg diet). CBZ and biotin-supplemented diets began on d 5 and 26,
respectively, and continued through d 68. Rats (n = 5/group) were killed
on d 5, 26, 47 or 68. CBZ reduced serum and liver free biotin (P <
0.05), whereas biotin supplementation during CBZ administration
maintained biotin status. CBZ also decreased specific activities and
abundance of biotinylated pyruvate and acetyl CoA carboxylases (PC and
ACC, P < 0.05) in brain and liver, whereas biotin supplementation
prevented these decreases for ACC. Specific activity of PC was
maintained upon biotin supplementation, but the abundance of
biotinylated PC remained significantly decreased. Brain and serum
lactate were elevated after 68 d of CBZ treatment and were reduced to
control lactate concentrations upon biotin supplementation (P < 0.05).
Conversion of lactate to pyruvate and simultaneous generation of NADH
during biotin supplementation could explain how increases in PC activity
occur without changes in the abundance of biotinylated PC because we
found NADH to be an activator of PC activity in vitro. These results
support the use of biotin supplementation as a concurrent strategy
during CBZ administration to help maintain biotin status, function of
biotin-dependent enzymes and decrease CBZ-induced lactate accumulation.
PMID: 12949377 [PubMed - indexed for MEDLINE] |
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