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Science Forum Index » Life Extension Forum » antioxidant activity of choto-san and its related constitue
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 Posted: Thu Jan 08, 2004 7:31 pm |
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Biol Pharm Bull. 2004 Jan;27(1):38-46.
Antioxidant and Free Radical-Scavenging Activity of Choto-san and Its
Related Constituents.
Mahakunakorn P, Tohda M, Murakami Y, Matsumoto K, Watanabe H.
Department of Pharmacology, Institute of Natural Medicine, Toyama
Medical and Pharmaceutical University.
The antioxidant properties of Choto-san and its related constituents
such as Chotoko and Choto-san without Chotoko, and phenolic compounds
contained in Chotoko such as epicatechin, caffeic, acid and quercetin
were evaluated. In the 1,1-diphenyl-2-picrylhydrazyl (DPPH)
radical-scavenging assay, the scavenging activity of Chotoko (IC(50)
14.3 microg/ml) was found to be higher than that of Choto-san (IC(50)
206.2 microg/ml) and Choto-san without Chotoko (IC(50) 244.3
microg/ml). Epicatechin (IC(50) 10.4 microM), caffeic acid (IC(50)
13.8 microM), and quercetin (IC(50) 7.1 microM) also revealed
scavenging activity against DPPH radicals. Choto-san (IC(50) 67.7
microg/ml) exhibited stronger inhibitory activity against superoxide
anion formation than Choto-san without Chotoko (IC(50) 92.4 microg/ml)
but weaker activity than Chotoko (IC(50) 18.3 microg/ml). The
generation of superoxide anion was also inhibited by epicatechin
(IC(50) 175.2 microM), caffeic acid (IC(50) 141.7 microM), and
quercetin (IC(50) 18.7 microM). In a hydroxyl radical-scavenging
experiment, Choto-san (IC(50) 2.4 mg/ml), Chotoko (IC(50) 2.2 mg/ml),
Choto-san without Chotoko (IC(50) 2.8 mg/ml), epicatechin (IC(50) 3.9
mM), caffeic acid (IC(50) 3.6 mM), and quercetin (IC(50) 1.9 mM)
exhibited activity. In NG108-15 cells, when added simultaneously with
H(2)O(2) (500 microM), Choto-san (250 microg/ml), Chotoko (250
microg/ml), Choto-san without Chotoko (500 microg/ml), epicatechin
(200 microM), caffeic acid (200 microM), and quercetin (200 microM)
effectively protected cells from oxidative damage. In conclusion, the
present results provide evidence that Choto-san acts as an antioxidant
and cytoprotective agent against oxidative damage, which is due at
least partly to the phenolic compounds contained in Chotoko.
PMID: 14709896 [PubMed - in process]
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=14709896&dopt=Abstract |
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